Ostreococcus

Sampling of Ostreococcus transformants in semi-solid agarose medium
Description: 

Ostreococcus tauri (Prasinophyceae, Mamiellales) belongs to eukaryotic picophytoplankton. The genus Ostreococcus has a worldwide distribution and ecologically significant contribution in several marine ecosystems. Discovered in the Thau Lagoon, O. tauri has been described as the smallest free-living eukaryote. The cellular ultrastructure of Ostreococcus sp is extremely simple and the genome is very compact with little gene redundancy. The implementation of techniques of functional genomics based on genetic transformation, have recently promoted O. tauri a new model to study conserved biological functions such as the circadian clock and more generally responses to environmental stresses.

Ostreococcus tauri

Plantae, Chlorobionta, Chlorophyta, Prasinophyceae, Mamiellale.

 

Genetic resources: 

Available data

Data Experiment Size Version
Sequence   12,5 Mb  
Transcriptome RNA-seq Yes  
Transcriptome MicroArray Yes 27 samples
ESTs   Yes  
Annotation   Yes 2.1
SNP   Yes  
Ecotypes   Yes  
Proteomic   Yes  

Genome is available on picoplaza (part of the Gent University website). There is a 2.1 version in restricted acces which integrate latest RNA-seq data and annotation in Orcae.

Available individuals

There is 10.000 strain archived (15.000 insertions). They are under sequencing so edge data will be available in future. There is no phenotyping in progress.

Toolbox

Plasmids :

  • pOtox vector, FN554877
  • pOtLuc vector, FN54878
  • pOtoxLuc vector

Maps available :

  • Corellou et al., Clocks in the green lineage: comparative functional analysis of the circadian architecture of the picoeukaryote ostreococcus, Plant Cell 2009
  • Moulager et al.,Integration of light signals by the retinoblastoma pathway in the control of S phase entry in the picophytoplanktonic cell Ostreococcus, PLoS Genetics 2010

 

Culture: 

Standard culture medium based on Keller or artificial seawater type (Djouani Tahri et al., A Phosphate-Regulated Promoter for Fine-Tuned and Reversible Overexpression in Ostreococcus: Application to Circadian Clock Functional Analysis, PloS one 2011)
 

Contact: 

François-Yves Bouget, Laboratoire d'Océanographie Microbiologique, UMR 7621, Observatoire Océanologique Laboratoire Arago, BP 44 66651 Banyuls-sur-Mer